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Flowjo tutorial3/26/2023 The matching algorithm compares the imported sample from the FlowJo workspace or external analysis archive against previously imported FCS files using the following properties and keywords: FCS file name or FlowJo sample name, $FIL, GUID, $TOT, $PAR, $DATE, $ETIM. When importing analysis results from a FlowJo workspace or an external analysis archive, the Flow Module will attempt to find a previously imported FCS file to link the analysis results to. When the import process completes, you will see a datagrid named "labkey-demo.xml." To learn how to customize this datagrid to display the columns of your choice, see this topic: Step 1: Customize Your Grid View.While the job runs, you will see the current status file growing and have the opportunity to cancel if necessary using the "Cancel Job" button. Review the properties and click Finish to import the workspace.If the flow metadata includes PTID and Date/Visit columns matching those in the study, information from the study will be included in the FCSAnalyses table. (Optional) Choose a target study folder.Accept the default name of your analysis folder, "Analysis".Confirm that all samples (= 68 samples) are selected and click Next.See below for more on the exact algorithm used to resolve the FCS files. To manually correct any mistakes, select the appropriate FCS file from the combobox next to the sample's name. If you were importing samples that matched existing FCS files, such as reimporting a workspace, they would have a green checkmark and unmatched samples would have a red checkmark. The import wizard will attempt to match the imported samples from the FlowJo workspace with the previously imported FCS files.In the right panel, check the box for the FACSData folder. Click the "labkey-demo" folder to open it.Select Browse the pipeline for the directory of FCS files.For the sake of this tutorial, you can safely ignore these warnings. If this were real data and you were required to import these statistics, you would have to go back to FlowJo, re-calculate the missing statistics, and then save as xml again. We have intentionally incorporated these warnings to illustrate the point that if the FlowJo workspace is missing calculations for any statistics, those values won't be imported. While all user data is archived by the shared resource, there may be a delay in accessing data files that were generated longer than one year ago.Sample 118756.fcs (286): 118756.fcs: S/L/-: Count statistic missing To expedite access to your data, we suggest that you retrieve data shortly after it is generated and store it in a secure place. Contact us for shared drive access permission. Just copy your data to the share drive (or ask us to do it) and download the data to your server. We also have a shared-access drive for data transfer purposes. The FCSR archives data for our users for up to 7 years. Modfit Cell Cycle Analysis – Staff Assisted.Astrolabe – Cloud-Based Data Analysis and Bioinformatics Available for Purchase ( Video Tutorial ) ( Info Flyer).FCS Express – Site License Available for Purchase ( Video Resources).
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